P-631 Alleviating effects of EGCG on ovarian hyperstimulation syndrome and the potential modulating mechanism through the VEGF pathway

Abstract Study question What are the underlying mechanisms of EGCG to alleviate Ovary hyperstimulation syndrome (OHSS)? Summary answer inhibited VEGF via TGF-β1 classical-SMAD pathway and 67LR-mediated CREB could reduce ovarian inflammatory effect attenuated OHSS progress in rat model. is known already Ovarian (OHSS) one most severe complications COH during IVF treatment. The pathophysiology characterized by increased capillary permeability, an important mediator OHSS, serum levels have been shown correlate with severity. (-)-epigallocatechin-3-gallate (EGCG) abundant biologically active polyphenolic catechin green tea has reported multiple effects humans. Many research indicated that many pathological processes, inhibit its receptor expression angiogenesis effect. Herein, might a therapeutic on OHSS. design, size, duration We investigated role vitro vivo. primary human granulosa-lutein(hGL) cells granulosa-like tumor (KGN) cell line were cultured treated different concentrations for 24 hours. Animal model was established SD rats injection pregnant mare gonadotropin (PMSG), randomly assigned receive vehicle only or 3 days. All experiments performed 3-8 times comparisons. Participants/materials, setting, methods KGN hGL determined MTT assay. body weight measured every day, ovary size after removal, permeability Evans-blue. estrogen level detected ELISA. RNA protein expressions VEGF, VEGFR-2, TGF-β, TβR II qPCR Western-blotting immunostaining vivo experiments. Main results chance Our study demonstrated administration development rats, as histological examination morphology. significantly decreased treatment group compared (147.9 vs 206.5 mg). Additionally, exhibit downregulated IHC RT-qPCR. E2 decrease group. exerted inhibitory growth high dose (50uM) longtime (48h) cells. In cells, reduces TGF-β at levels. Furthermore, inhibits TGF-β1-induced production secretion suppressing traditional Smad signaling pathway. also downregulates through 67-kDa laminin receptor-mediated PKA-CREB Limitations, reasons caution employ wide range biological effects. Therefore, suppressive be part pharmacological syndrome. Further studies needed explore melatonin from other perspectives. Wider implications findings add mechanistic insight support using adjuvant therapy management shows potential act novel alternative drug treat Daily intake beneficial women under prevent Trial registration number Not applicable